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The amount of membrane protein and WGA-SPA bead depended on the expression density and was 0.5–1.0 μg protein with 0.25 mg bead for the high-, 1.5–7.5 μg protein with 0.25–0.5 mg bead for the medium-, and 5.0–20 μg protein with 0.25–0.75 mg bead for the low-expressing cell lines
